rabbit anti zo 1 tjp1 antibody  (Bioss)

Journal: Neuropsychiatric Disease and Treatment

Article Title: Antidepressant-Like Effects of Mongolian Medical Warm Acupuncture via Remodeling the Gut Microbiota–Metabolite–Barrier Axis in CUMS Rats

doi: 10.2147/NDT.S566386

Figure Lengend Snippet: Warm acupuncture enhances intestinal barrier integrity and suppresses histidine metabolism in CUMS rats. ( A – C ) mRNA expression levels of tight junction–related genes Occludin, Tjp1 (ZO-1), and Claudin4 in colonic tissues, determined by RT-qPCR. ( D ) Representative Western blot and quantitative analysis of Occludin, Tjp1, and Claudin4 protein expression in the colon. ( E ) mRNA expression of histidine metabolism–related enzymes HAL and HDC, showing upregulation in CUMS rats and suppression by MMWA intervention. Data are presented as mean ± SD (n = 3–6/group). * p < 0.05, *** p < 0.001 vs Control; # p < 0.05, ## p < 0.01, ### p < 0.001 vs CUMS.

Article Snippet: Membranes were blocked and incubated overnight at 4 °C with primary antibodies against Occludin (1:1000, Abcam, ab216327), TJP1 (ZO-1, 1:1000, CST, # 13663), Claudin4 (1:1000, Abcam, ab15104), HAL (1:500, Proteintech, 15756-1-AP), HDC (1:500, Santa Cruz, sc-271213), and β-actin (1:2000, Abcam, ab8226).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Control